Phytochemical Screening and Hplc Analysis of Flavonoid and Anthraquinone Glycoside in Zanthoxylum Armatum Fruit

Objective: The present paper deals with the phytochemical screening of Zanthoxylum armatum fruits, an important medicinal plant of J&K region. This study involves the preliminary screening. Further HPLC flavonoid and glycoside profile of the methanolic extract had been studied. Methods: The retention behavior of flavonoid and glycoside isolated in methanolic extract of zanthoxylum armatum fruits in a reversed-phase (RP) high performance liquid chromatography (HPLC) system has been studied on a C18-RP column using acetonitrile–water and methanol–water mixtures as mobile phase. Results: The preliminary screening test results in the detection of bioactive principles and the retention time is identified in the HPLC chromatogram, as flavonoid (Rt=2.8) and anthraquinone (Rt=2.2). Conclusion: The generated data has provided the basis for its wide uses as the therapeutic in the traditional and folk medicine.